586 Interest of Silybum marianum extract, Manganese PCA and Lespedeza capitata extract in a new anti-hair loss serum to stimulate hair growth and prolong anagen phase

The hair follicle (HF) undergoes cycles of growth (anagen), regression (catagen) and rest (telogen) phases. Chronic reactive loss are linked to dysfunctions the cycle. association active ingredients targeting specifically HF could help restore cycle promote growth. efficacy 3 was evaluated on growth/anchorage markers in human dermal papilla cells (DPC): a new extract from Silybum marianum (SME) stimulating shaft anchorage by keratin 75 modulation, Manganese PCA (MnPCA) Lespedeza capitata (LCE). Antibody array gene reporter assays allowed analyse receptor tyrosine kinase phosphorylation Wnt/β catenin pathway. ELISA tests were performed quantify release Versican, VEGF DKK1. 5α-reductase (5αR) activity also measured. anti-hair serum containing these then ex vivo full-thickness scalp skin model investigate its ability improve In DPC model, SME activated factor signalling pathways (EGFR x1.9 PDGFR x2.8 respectively) their downstream effectors (ERK, GSK3, Akt, STAT x1.2 2). MnPCA induced Versican (x33) (x3.3) secretion, but (+80%). LCE reduced DKK1 (-72%) 5αR (-60%). explant stimulated shafts production (+102%) significantly prolonged anagen phase improving score matrix keratinocytes proliferation. Thus, SME, displayed complementary properties. interest combining confirmed using that improved phase.